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There's no established protocol

It is especially useful where oral intake is insufficient or absorption is compromised, as noted in the product literature

Giorgio Roberto Merlo, University of Turin, Italy Updates Copyright 2021 Desole, Gallo, Vitacolonna, Montarolo, Bertolotto, Vivien, Comoglio and Crepaldi

When the thyroid is not functioning properly, there may be an excess or lack of thyroid hormone production, which can lead to hyper or hypothyroidism, respectively

Klotho levels decline significantly with age: young adults (18-35) have the highest levels, with progressive decreases through middle age and into older adulthood