The PCR products were purified using magnetic beads (Axygen Biosciences, Union City, CA, USA), and the concentration of the amplicon library was estimated using a 2100 Bioanalyzer System (Agilent Technologies Inc., Waldbronn, Germany)
To reducing the carryover in method, we have developed a binary flow method in HPLC with gradient adjustment from 5% to 90% organic phase, where we have observed reducing carryover and improving LLOQ signal in development phase
Chinese water-pipe smoking and the risk of COPD
Long-term studies should assess the impact on offspring health and development
It was first isolated and characterised by Professor Predrag Sikiric and his research group at the University of Zagreb in the early 1990s, with the foundational paper published in the Journal of Physiology (Paris) in 1993