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Briefly, 5 L serum (after tenfold dilution with saline) was added to the total reaction system containing GSSG, NADPH and DTNB (5,5-dithiobis-(2-nitrobenzoic acid)

Consistent with human observations, H2O2 also promoted FOXO4 nuclear translocation in TM3 cells, along with an increase in senescence markers p53, phospho-p53, and p21, while reducing testosterone synthesis-related enzymes like CYP11A1 and CYP17A1

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J Biol Chem 179:12451254 Carmel-Harel O, Storz G (2000) Roles of the glutathione- and thioredoxin-dependent reduction systems in the Escherichia coli and Saccharomyces cerevisiae responses to oxidative stress