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The possible association of MEL with hepatic steatosis is based on historical observations that showed that pinealectomy increased serum glucose levels in laboratory animals, while administration of MEL corrected these abnormalities (100, 101)

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Imaging and figure preparation Images of hypothalamic cultures were collected on the calcium imaging microscope as described above, and separate cultures were plated onto PhenoPlateTM 96-well microplates (Perkin Elmer), fixed in 4% paraformaldehyde (Thermo Fisher Scientific) for 10 minutes, rinsed three times with Tris-buffered saline containing 0.1% Triton X-100 (TBS-T

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