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Biomarker Assessment and Provider-Guided Decision-Making Before considering glutathione supplementation, qualified healthcare providers typically assess baseline detoxification capacity through biomarkers: plasma glutathione levels, glutathione S-transferase activity, homocysteine and methylmalonic acid (reflecting transsulfuration and B12 status), and inflammatory markers like high-sensitivity CRP or cytokine panels

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Later, this methodology was adapted and used to study other epithelia of different organs including the GI tract

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The laccase activity was measured by adding 180 l of reaction mixture (100 mM citratephosphate buffer pH 4.0 and 1 mM ABTS) by using a Multidrop robot (Multidrop Combi, Thermo Scientific)