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While our qPCR analysis relied on a single reference gene (GAPDH) that showed stable expression, future studies could benefit from incorporating multiple reference genes as recommended by the MIQE guidelines [51]

Enhanced hepatic lipid accumulation due to increased adipose tissue-linked lipolysis, especially in conditions of obesity, remains the hallmark of NAFLD

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The evidence base is almost entirely preclinical: rodent models consistently show accelerated tissue repair, gut healing, and neurological protection, but human RCT data remains largely absent