Once absorbed, GHK-Cu acts as a signaling peptide, supporting the skin's natural renewal processes and helping maintain a healthy, youthful-looking complexion
[DOI] [PubMed] [Google Scholar] Davis JF, Schurdak JD, Magrisso IJ, Mul JD, Grayson BE, Pfluger PT, Tschp MH, Seeley RJ, Benoit SC, 2012
But timing isn't optional if you want maximum efficacy
The anti-apoptotic effect was attenuated by PKA inhibition or GLP-1-R knockdown, confirming its dependence on GLP-1-R-PKA/-catenin signaling
Transfection HEK293 cells were transfected with purified DNAs including pEGFP-C1, pEGFP-N3-MIM I-BAR, pHalo7-C1, pHalo7-C1-MIM I-BAR, pEGFP-C1-GST-Rac1, pHalo7-C1-Rac1 WT and mutants (G12V and T17N), pmCherry-FKBP-MIM I-BAR, pQH-CD63-sfGFP-FKBP, pKLV2-AsCas12fHKRA-Halo7-GSGS-FRB-guideRNA, and pKLV2-AsCas12fHKRA-Halo7-GSGS-FRB-T2A-FKBP-MIM I-BAR-guideRNA using polyethylenimine MAX (PEI, Polysciences, #49553-93-7)