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After washing three times, cells were resuspended in 200 L PBS containing 1% BSA for detection by NovoCyte (Agilent) utilizing laser excitation and emission wavelengths of 488 nm and 530 nm, respectively

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Cytokine treatment induced a 1.7-fold and 2.2-fold increase in dead-cell protease and caspase 3 activity, respectively, compared with control treatment (Fig

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