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FNDC5 Attenuates Adipose Tissue Inflammation and Insulin Resistance via AMPK-Mediated Macrophage Polarization in Obesity

The result was a milky filtrate that was let to sit for 48 h after being passed through a cheesecloth sieve

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After removing the magnetic beads, samples were spun down at 20,000 g for 10 min to remove the remaining beads, and the supernatant was transferred to a scintillation vial with 5 mL of Insta-Gel Plus scintillation cocktail (Perkin Elmer #601339)

Chemical Synthesis Reaction Equation of Carnitine Chemical Synthesis Process Flow of Carnitine Purification Method: Enzymatic conversion is employed