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Treatment of Drosophila flies with kynurenine pathway inhibitors and the NAD + precursors NR or NAM increased the lifespan of these animals, suggesting an advantage in combining different therapeutic strategies

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An amount of 50 ml of 1 M Butanolic HCl was added to each sample and incubated at 60C for 15 min

Retro-transcriptions were processed, as described in the method section, on 2 g of RNA by doubling the total reaction volume (40 l final)