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Transmission electron microscopy (TEM) analysis of mouse cells Islets were mounted in 1% agarose, fixed in 0.1 M PBS containing 2% glutaraldehyde for 12 h at 4 C, and refixed in 1% osmium tetroxide in 0.1 M PBS for 2 h at room temperature

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Nevertheless, spexin exemplifies a peptide whose underexplored status reflects concerns about pleiotropy rather than a lack of metabolic relevance