Vitamin B-12 is a water-soluble B complex vitamin found naturally in a variety of foods, including meat, fish and dairy products
Vitamin B12 injections may also benefit people with certain types of cancer, low levels of stomach acid, or alcohol withdrawal
Reported applications include NNMT enzyme inhibition assays measuring 1-methylnicotinamide formation or S-adenosylhomocysteine release, intracellular target-engagement assays in differentiated 3T3-L1 adipocytes where 5-Amino-1MQ reduced intracellular 1-methylnicotinamide with an EC near 2.3 M (Neelakantan et al., Biochem Pharmacol, 2018), membrane permeability and Caco-2 transport characterisation, methyltransferase selectivity panels covering COMT, DNMT1 and PRMT3, and rodent studies of NNMT inhibition in adipose tissue and liver following the antisense knockdown work of Kraus and colleagues (Nature, 2014)
The injection is given directly into the muscle, typically in the thigh or upper arm
The exact dosage is dependent on medical condition, response to the treatment, age, and weight